| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 28.0 kDa, a disulfide-linked homodimeric protein containing two 129 amino acids. |
| AA Sequence: |
TALAGTRTAQ GSGGGAGRGH GRRGRSRCSR KPLHVDFKEL GWDDWIIAPL DYEAYHCEGL CDFPLRSHLE PTNHAIIQTL LNSMAPDAAP ASCCVPARLS PISILYIDAA NNVVYKQYED MVVEACGCR |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/mL, corresponding to a specific activity of > 1000 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 30% acetonitrile and 0.1% TFA. |
| Endotoxin: |
Less than 0.1 EU/µg of rHuGDF-7/BMP-12 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Growth and differentiation factors (GDFs 1 through 15) belong to the BMP family within the TGF-beta superfamily of proteins. Initially synthesized as inactive preproproteins, they undergo cleavage and subsequent formation into active homodimers that are secreted. These dimers of GDFs are typically disulfide-linked, except for GDF-3 and GDF-9. During embryonic development, GDF proteins play crucial roles, especially in the development of skeletal, nervous, and muscular systems. |
