| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 8.6 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids. |
| AA Sequence: |
VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK EMSKRSP |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH7.4, 50 mM NaCl. |
| Endotoxin: |
Less than 1 EU/µg of rHuIP-10/CXCL10 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Gamma-IP10, Small-inducible Cytokine B10 |
| Background: |
CXCL10, also known as IP-10, is a member of the CXC chemokine family and is produced by the CXCL10 gene. Initially discovered in monocytes, endothelial cells, and fibroblasts, CXCL10 responds to IFN-γ and exerts its influence through the CXCR3 receptor on cell surfaces. It acts as a chemoattractant for activated T-lymphocytes and monocytes/macrophages, promotes T-cell adhesion to endothelial cells, and inhibits both bone marrow colony formation and angiogenesis. The mouse equivalent, Crg-2, shares roughly 67% amino acid sequence identity with its human counterpart. |
