| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 155 amino acids. |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using AML5 cells is less than 1 ng/mL. The specific activity of recombinant human Flt-3 Ligand GMP is > 1.0 × 106 Units/mg, which is calibrated against the rHuFlt-3 Ligand WHO reference reagent (NIBSC code: 96/532). |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered solution in PBS, pH7.0. |
| Endotoxin: |
Less than 0.01 EU/mg of rHuFlt-3 Ligand GMP as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
The Flt-3 ligand (FL) is a hematopoietic cytokine, a type of cell-signaling protein, recently found. Its functions are carried out through its attachment to the Flt-3 transmembrane glycoprotein. Flt-3 was initially discovered to be part of the class III subfamily of receptor tyrosine kinases (RTK), with its expression in hematopoietic cells observed to be limited to abundant stem/progenitor cell populations. The class III RTKs also encompass the receptors SCF, M-CSF, and PDGF. Consequently, Flt-3 ligand shares a structural resemblance to M-CSF and SCF. These three cytokines have been demonstrated to exist both as type I transmembrane proteins and as soluble proteins. The main isoform of human FL is a transmembrane protein, which can be broken down proteolytically to produce a soluble version of the protein. An alternative FL mRNA that produces a soluble variant of the human FL has also been found. FL is commonly found in a range of human and mouse tissues. When compared at the amino acid sequence level, human and mouse FL show about 72% similarity and the two proteins display cross-species functionality. FL is known to work in conjunction with a broad range of hematopoietic cytokines to spur the growth and differentiation of early hematopoietic progenitors. |
| Shipping: |
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Sequence: |
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA |
| Sterility: |
Negative. |
| Mycoplasma: |
Negative. |
| Host Cell Protein: |
Less than 0.05% when tested by ELISA. |
| Quality Statement: |
The manufacturing and testing of these products comply with ICH Q7 guidelines. |
| Host Cell DNA: |
Less than 20 ng/mg when tested by qPCR. |
| In Vitro Virus Assay: |
Negative. |
