| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 155 amino acids. |
| AA Sequence: |
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.0. |
| Endotoxin: |
Less than 1 EU/μg of rHuFlt3-Ligand as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Flt3L, SL Cytokine |
| Background: |
Flt3-ligand (FL) is a hematopoietic cytokine that binds to the Flt3 transmembrane glycoprotein. Flt3 is a class III receptor tyrosine kinase (RTK) found primarily in stem/progenitor cell populations within the hematopoietic system. This class of RTKs also includes receptors for SCF, M-CSF, and PDGF. FL, structurally similar to M-CSF and SCF, exists as both a type I transmembrane protein and a soluble protein. The main human FL isoform is a transmembrane protein that can be cleaved to produce a soluble form. An mRNA variant of FL also encodes a soluble version of the protein. FL is expressed in a variety of human and mouse tissues and shares about 72% amino acid sequence identity between the two species, allowing for cross-species activity. FL works in conjunction with other hematopoietic cytokines to stimulate the growth and differentiation of early hematopoietic progenitors. |
