| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids. |
| AA Sequence: |
GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, with 350 mM NaCl, 5% trehalose and 0.02% Tween-20, pH7.0. |
| Endotoxin: |
Less than 1 EU/µg of rHuFGF-4 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 500 mM NaCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3 |
| Background: |
FGF-4, also referred to as FGFK and KFGF, is a member of the fibroblast growth factor (FGF) family. It exerts its biological effects through signaling pathways involving FGF R1c, 2c, 3c, and 4 receptors. FGF-4 plays a crucial role in sustaining a population of progenitor cells within the epiblast, which subsequently gives rise to mesodermal tissues. Furthermore, it contributes to the stem cell population that becomes part of the tailbud. This factor is essential for the axial elongation of the mouse embryo post-gastrulation. The mature form of human FGF-4, comprising amino acids 71 to 206, exhibits significant sequence homology, sharing 91%, 82%, 94%, and 91% identity with its murine, rat, canine, and bovine counterparts, respectively. Additionally, FGF-4 shares approximately 30% sequence identity with the prototypical members of the FGF family, underscoring its evolutionary conservation and functional significance within this protein family. |
