| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 23.6 kDa, a single non-glycosylated polypeptide chain containing 206 amino acids. |
| AA Sequence: |
AEVGGVFASL DWDLHGFSSS LGNVPLADSP GFLNERLGQI EGKLQRGSPT DFAHLKGILR RRQLYCRTGF HLEIFPNGTV HGTRHDHSRF GILEFISLAV GLISIRGVDS GLYLGMNERG ELYGSKKLTR ECVFREQFEE NWYNTYASTL YKHSDSERQY YVALNKDGSP REGYRTKRHQ KFTHFLPRPV DPSKLPSMSR DLFHYR |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile colorless liquid. |
| Formulation: |
Supplied as a 0.2 μm filtered solution in 20 mM Tris-HCl, 1 M NaCl, pH 9.0, with 0.02% Tween-20, 10% glycerol. |
| Endotoxin: |
Less than 0.1 EU/µg of rHuFGF-16 as determined by LAL method. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70°C as supplied.- 3 months, -20 to -70°C under sterile conditions after opening. |
| Background: |
Fibroblast growth factor 16 (FGF-16) is a member of the extensive FGF family, characterized by heparin-binding properties and featuring a core 120 amino acid FGF domain that ensures a consistent tertiary structure across the family. Initially discovered in rat heart tissue through homology-based polymerase chain reaction, human FGF-16 cDNA encodes a 207 amino acid precursor protein with a single N-linked glycosylation site. Unlike conventional proteins, FGF-16 does not possess a typical signal peptide but is efficiently produced through alternative secretion pathways. Among FGF family members, FGF-16 bears the highest resemblance to FGF-9, exhibiting a 73% sequence homology at the amino acid level. Human FGF-16 shares a strikingly high sequence identity of 99% and 98.6% with mouse and rat FGF-16, respectively. |
