| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 27.6 kDa, a single non-glycosylated polypeptide chain containing 245 amino acids. |
| AA Sequence: |
MAAAIASSLI RQKRQARERE KSNACKCVSS PSKGKTSCDK NKLNVFSRVK LFGSKKRRRR RPEPQLKGIV TKLYSRQGYH LQLQADGTID GTKDEDSTYT LFNLIPVGLR VVAIQGVQTK LYLAMNSEGY LYTSELFTPE CKFKESVFEN YYVTYSSMIY RQQQSGRGWY LGLNKEGEIM KGNHVKKNKP AAHFLPKPLK VAMYKEPSLH DLTEFSRSGS GTPTKSRSVS GVLNGGKSMS HNEST |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH8.5, 500 mM NaCl. |
| Endotoxin: |
Less than 1 EU/µg of rHuFGF-13 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
FHF-2 |
| Background: |
The human FGF-13, encoded by the FGF13 gene, is a part of the FGF-11 subfamily, which includes four members: FGF-11 to FGF-14. Initially labeled as fibroblast growth factor homologous factors (FHFs), these members share significant sequence similarity with other FGFs but lack the ability to activate FGF receptors (FGFRs), thus were not typically classified as part of the FGF family. FGF-13 assumes a critical role in neuron polarization and migration within the cerebral cortex. In murine studies, FGF-13 RNA was detected in developing cells of the central nervous system, as well as widely distributed throughout the peripheral nervous system. |
