| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 71 amino acid residues. |
| AA Sequence: |
TRGSDISKTC CFQYSHKPLP WTWVRSYEFT SNSCSQRAVI FTTKRGKKVC THPRKKWVQK YISLLKTPKQ L |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CCR3 transfected HEK293 cells is in a concentration range of 0.5-2.0 μg/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuEotaxin-3/CCL26 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
CC chemokine IMAC, MIP-4-alpha, TSC-1 |
| Background: |
CCL26, a recently discovered chemokine of the CC family, is also referred to as TARC (thymus and activation-regulated chemokine). Encoded by the CCL26 gene located on chromosome 7 in humans, it is expressed at low levels throughout various tissues such as the heart and ovary. CCL26 exerts its biological effects by binding to the cell surface receptor CCR3, functioning as a chemoattractant for eosinophils and basophils. |
