| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.3 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids. |
| AA Sequence: |
TLQKKIEEIA AKYKHSVVKK CCYDGACVNN DETCEQRAAR ISLGPRCIKA FTECCVVASQ LRANISHKDM QLGR |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHuC5a as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Complement C5, also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, is a crucial part of the human complement system and is encoded by the C5 gene. Upon activation, Complement C5 is split into two fragments: C5a and C5b. C5a is essential for chemotaxis, attracting immune cells to sites of infection, while C5b initiates the formation of the complement membrane attack complex (MAC), crucial for cell lysis. This protein, the fifth component of the complement system, is integral to both inflammatory responses and cell destruction. Complement C5 consists of alpha and beta polypeptide chains connected by a disulfide bond. The alpha chain undergoes cleavage by a convertase enzyme, releasing C5a, an anaphylatoxin with strong spasmogenic and chemotactic properties. The remaining C5b fragment combines with the C6 complement component, setting the stage for the assembly of the MAC, which involves additional complement proteins. |
