| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids. |
| AA Sequence: |
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/mL, corresponding to a specific activity of > 6.7 × 103 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuCNTF as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
CNTF |
| Background: |
Ciliary Neurotrophic Factor (CNTF) is a polypeptide hormone which primarily functions within the nervous system, where it aids in the synthesis of neurotransmitters and the growth of neurites in specific neuronal populations. It was first discovered as a key growth factor for embryonic chick ciliary parasympathetic neurons in laboratory culture. CNTF further serves as a crucial survival factor for neurons and oligodendrocytes and plays a potential role in lessening tissue damage during inflammatory episodes. Additionally, CNTF's possible applications in treating motor neuron diseases and reducing food intake without provoking hunger or stress have been identified. In terms of structure, CNTF is linked to IL-6, IL-11, LIF, and OSM, with all these four-helix bundle cytokines sharing gp130 as a common subunit that transduces signals in their receptor complexes. Recombinant human CNTF, comprises 200 amino acids, and shares a sequence identity of 82% and 83% with mouse and rat CNTF respectively. |
