| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids. |
| AA Sequence: |
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered solution in PBS, pH7.0, with 5% Trehalose, 0.02% Tween-20. |
| Endotoxin: |
Less than 0.1 EU/μg of rHuCNTF as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Ciliary neurotrophic factor (CNTF) is a polypeptide hormone primarily active within the nervous system, where it enhances neurotransmitter synthesis and neurite outgrowth in specific neuronal populations. It was first identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Moreover, CNTF serves as a vital survival factor for neurons and oligodendrocytes and may help mitigate tissue damage during inflammatory episodes. Additionally, CNTF is beneficial in the treatment of motor neuron disease and can reduce food intake without causing hunger or stress. Structurally, CNTF is related to IL-6, IL-11, LIF, and OSM; all of these four-helix bundle cytokines utilize gp130 as a signal-transducing subunit in their receptor complexes. Recombinant human CNTF consists of 200 amino acids and shares 82% and 83% amino acid sequence identity with mouse and rat CNTF, respectively. |
