| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 11.2 kDa, a single, non-glycosylated polypeptide chain containing 101 amino acids. |
| AA Sequence: |
ANHHKAHHVK TGTCEVVALH RCCNKNKIEE RSQTVKCSCF PGQVAGTTRA APSCVDASIV EQKWWCHMQP CLEGEECKVL PDRKGWSCSS GNKVKTTRVT H |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity is determined by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. rHuTAFA-2, immobilized at 6-24 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuTAFA-2 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
FAM19A2 |
| Background: |
TAFA-2, also known as FAM19A2, is a chemokine-like protein belonging to the FAM19/TAFA family. This family, distantly related to MIP-1α, comprises five members and is newly discovered. TAFA proteins are notably expressed at high levels in specific brain regions. Like other TAFA family members (excluding TAFA5), mature TAFA1 contains 10 cysteine residues evenly spaced. Human TAFA2 shares 97% amino acid identity with mouse TAFA2. The biological functions of TAFA family members have yet to be fully elucidated, but they are hypothesized to act as brain-specific chemokines or neurokines involved in regulating immune and nervous system cells. |
