| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 21.2 kDa, a single non-glycosylated polypeptide chain containing 201 amino acids. |
| AA Sequence: |
MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/mL, corresponding to a specific activity of > 1.0× 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA. |
| Endotoxin: |
Less than 0.1 EU/µg of rHuCT-1 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Cardiotrophin-1 (CT-1) belongs to the cytokine family, which includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a versatile cytokine expressed in various tissues, such as the adult heart, skeletal muscle, ovary, colon, prostate, and fetal lung. Research indicates that CT-1 induces hypertrophy in cardiac myocytes in vitro by binding to and activating the ILST/gp130 receptor. The human CT-1 gene encodes a protein of 201 amino acids, lacking a hydrophobic signal peptide, and shares 78% and 80% amino acid sequence identity with murine and rat CT-1, respectively |
