| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 15.7 kDa, a monomeric, non-glycosylated polypeptide chain containing 139 amino acids. |
| AA Sequence: |
STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDL GWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP KPCCAPTQLN AISVLYFDDS SNVILKKYRN MVVRACGCH |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Data is not available. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 30% acetonitrile, 0.1% TFA. |
| Endotoxin: |
Less than 1 EU/μg of rHuBMP-7 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
OP-1 |
| Background: |
Bone Morphogenetic Protein 7 (BMP-7), part of the BMP group which includes members of the TGF-beta superfamily (BMP2-7), is involved in stimulating the formation of cartilage and bone. More than thirteen BMPs have been identified to date. BMP-7, primarily found in the kidney and bladder, is also present in the developing eyes, brain, and ear during embryogenesis. Also known as osteogenic protein-1 (OP-1), BMP-7 is a powerful cytokine that influences osteoblast differentiation, SMAD1 production, and the development and repair of the kidneys. Human BMP-7 is produced with a signal sequence, a propeptide, and a growth factor domain. The growth factor domain of human BMP-7 is almost identical to that of mouse and rat BMP-7, with 98% amino acid sequence identity. |
| Applications: |
1. Molecular standard (Western, ELISA) in studying secreted BMP-7;2. Preparing antibodies for BMP-7 monomer;3. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE. |
