| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 13.3 kDa, a monomeric, non-glycosylated polypeptide chain containing 117 amino acids. |
| AA Sequence: |
MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR |
| Purity: |
> 95% by SDS-PAGE. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 50 mM Na2CO3, 5 mM DTT, 5% trehalose, 0.02% Tween 20, pH11.0. |
| Endotoxin: |
Less than 1 EU/µg of rHuBMP-4 as determined by LAL method. |
| Reconstitution: |
Prior to opening, it is recommended to centrifuge the vial briefly to bring the contents down the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
A minimum of 12 months from date of receipt, when stored at ≤ -20°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
BMP-2B |
| Background: |
Bone morphogenetic protein 4, also known as BMP-4, is a member of the BMP family which belongs to the TGF-beta superfamily along with BMP2-7. There are over thirteen BMPs that have been identified and they play a critical role in the formation of cartilage and bone. BMP-4 is predominantly expressed in the lung, with lower levels found in the kidney. It is also present in both normal and cancerous prostate tissues, as well as in prostate cancer cell lines.
BMP-4 is involved in the development of teeth, limbs, and bone from mesoderm. Additionally, it plays a crucial role in the repair of fractures. BMP-4 signals through tetrameric complexes composed of type I and type II receptors and its function is regulated through interactions with various proteins and glycosaminoglycans. The human BMP-4 shares a 98% sequence identity with mouse BMP-4.
Decreased expression of BMP-4 has been linked to several bone disorders, including the hereditary condition Fibrodysplasia Ossificans Progressiva. |
| Applications: |
Molecular standard (Western, ELISA) in studying secreted BMP-4;
Preparing antibodies for BMP-4 monomer;
Molecule standard in detecting secreted BMP-4 in reduced SDS-PAGE. |
