| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 24.8 kDa, a homodimeric protein consisting of two 110 amino acid non-glycosylated polypeptide chains. |
| AA Sequence: |
QWIEPRNCAR RYLKVDFADI GWSEWIISPK SFDAYYCSGA CQFPMPKSLK PSNHATIQSI VRAVGVVPGI PEPCCVPEKM SSLSILFFDE NKNVVLKVYP NMTVESCACR |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit BMP-2-induced activity in murine MC3T3‑E1 cells. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 30% acetonitrile and 0.1% TFA. |
| Endotoxin: |
Less than 1 EU/µg of rHuBMP-3 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Bone morphogenetic protein 3 (BMP-3) is part of the BMP family, a subset of the TGF-beta superfamily that includes BMP2-7. Over thirteen BMPs have been identified to date, playing crucial roles in stimulating the formation of cartilage and bone. Initially recognized as regulators of skeletal tissues, BMPs are now known to contribute to the development and structural organization of various tissues and organs during embryogenesis. They also govern processes such as cell growth, differentiation, chemotaxis, and apoptosis across different cell types. Like other members of the TGF-beta family, BMPs exhibit significant conservation across animal species, with mature human and rat BMP-3 sharing 98% amino acid sequence identity. |
