| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 26.0 kDa, a homodimeric protein consisting of two 115 amino acid non-glycosylated polypeptide chains. |
| AA Sequence: |
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/mL, corresponding to a specific activity of > 5.0 × 103 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution containing 10 mM sodium citrate pH3.5. |
| Endotoxin: |
Less than 1 EU/µg of rHuBMP-2 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute in sterile distilled H2O to a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
BMP-2A |
| Background: |
Bone Morphogenetic Protein 2 (BMP-2), another member of the BMP group and the TGF-beta superfamily (BMP2-7), is implicated in the induction of cartilage and bone formation. Over thirteen BMPs have been discovered, with BMP-2 being expressed in various tissues such as the lung, spleen, brain, liver, prostate, ovary, and small intestine. BMP-2 is a potent cytokine that works in conjunction with osteoconductive carriers like collagen and synthetic hydroxyapatite to stimulate bone production. Recombinant human BMP-2 is used in orthopedics and dentistry. BMP-2 functions as a disulfide-linked homodimer with a molecular weight of approximately 26 kDa. The precursor of BMP-2 consists of 259 amino acids, including a signal sequence that is removed by a furin-type protease to produce the mature peptide. |
