| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids |
| Purity: |
> 98% by SDS-PAGE analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 U/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, 150 mM NaCl, 5% Trehalose, 0.02% Tween-20, pH7.6. |
| Endotoxin: |
Less than 0.01 EU/µg of rHubFGF GMP as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-0.3 mg/mL. If animal-origin-free condition is expected in your product, then sterile distilled water is recommended. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Human Basic Fibroblast Growth Factor (bFGF), a product of the FGF2 gene, is a component of the broader fibroblast growth factor family. Discovery of this growth factor began with the detection in pituitary extracts in 1973, leading to studies that revealed its ability to cause the expansion of fibroblasts. Following further experimentation with the extract using various pHlevels, two distinct forms were separated designated as "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). The human bFGF shares 54% of its amino acid sequence with aFGF. The bond between bFGF and its receptors can be enhanced with heparin or heparan sulfate proteoglycan. This growth factor is crucial in controlling cell survival, division, angiogenesis, differentiation, and migration. It is involved in numerous biological actions such as embryonic development, tissue repair, morphogenesis, tumor growth and invasion. Additionally, bFGF is often employed as a key element in cell culture mediums, like human embryonic stem cell culture medium and serum-free culture systems. |
| Shipping: |
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Sequence: |
MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS |
| Sterility: |
Negative. |
| Mycoplasma: |
Negative. |
| Host Cell Protein: |
Less than 0.05% when tested by ELISA. |
| Quality Statement: |
The manufacturing and testing of these products comply with ICH Q7 guidelines. |
| Host Cell DNA: |
Less than 20 ng/mg when tested by qPCR. |
| In Vitro Virus Assay: |
Negative. |
