| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 11.3 kDa, a single non-glycosylated polypeptide chain containing 98 amino acid residues. |
| AA Sequence: |
SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is between 5-10 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rHuAmphiregulin as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
AR, AREG, Colorectum Cell-derived Growth Factor, CRDGF |
| Background: |
Amphiregulin, an EGF related growth factor, was first isolated from MCF-7, a human breast carcinoma cell line that had been treated with PMA. Main expressions of Amphiregulin occur in numerous carcinoma cell lines and various human tissue epithelial cells like those in the colon, stomach, breast, ovary, kidney. The mature protein is released from Amphiregulin's extracellular domain which was initially synthesized as a transmembrane protein. Amphiregulin has 6 cysteine residues conserved, forming 3 intramolecular disulfide bonds vital to biological activity. Amphiregulin signals through the EGF/ TGF-a receptor, stimulating keratinocytes, epithelial cells and some fibroblasts growth while inhibiting certain carcinoma cell lines' growth. Skin phenotype resembling psoriasis is associated with mutations in the protein encoded by this. |
