| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids. |
| AA Sequence: |
ERTRSLQDPC SNCPAGTFCD NNRNQICSPC PPNSFSSAGG QRTCDICRQC KGVFRTRKEC SSTSNAECDC TPGFHCLGAG CSMCEQDCKQ GQELTKKGCK DCCFGTFNDQ KRGICRPWTN CSLDGKSVLV NGTKERDVVC GPSPADLSPG ASSVTPPAPA REPGHS |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity is determined by its inhibitory effect of IL-8 production using human peripheral blood mononuclear cells. About 90% of inibition was seen using a concentration of 1 µg for both 4-1BB Ligand and 4-1BB Receptor. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH8.0, 150 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHu4-1BB Receptor as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
TNFRSF9, CD137 Antigen, T-cell Antigen ILA |
| Background: |
The 4-1BB receptor, part of the TNF receptor family and also known as TNFRSF9, is predominantly present on T cells but also appears on B cells, monocytes, and various cell lines. It interacts with 4-1BBL to enhance the activity of activated T cells. This receptor is involved in presenting antigens and producing cytotoxic T cells. The crosslinking of the 4-1BB receptor boosts T cell growth, IL-2 secretion, survival, and cytotoxic capabilities, which can help eradicate tumors in mice. |
