| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 184 amino acids. |
| AA Sequence: |
REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by stimulation of IL-8 production using human PBMC is less than 10 ng/mL, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rHu4-1BBL as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
TNFSF9, CD137L |
| Background: |
4-1BBL, belonging to the TNF receptor family, aids in the proliferation, survival, and maturation of T cells. It is also expressed on several immune cells, including dendritic cells and natural killer cells, and is found at inflammation sites. The human 4-1BBL gene encodes a type II transmembrane protein with a significant extracellular domain, sharing a moderate level of amino acid identity with its mouse and rat counterparts. |
