| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 12.6 kDa, a single non-glycosylated polypeptide chain containing 119 amino acids. |
| AA Sequence: |
MGGWSSKPRQ GMGTNLSVPN PLGFFPDHQL DPAFGANSNN PDWDFNPNKD HWPEAHQVGA GAFGPGFTPP HGGLLGWSPQ AQGILTTVPV APPPASTNRQ SGRQPTPISP PLRDSHPQA |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 50 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rHBsAg-preS1 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- Refer to lot specific COA for the Use by Date when stored at ≤ -20°C as supplied.- 1 month, 2 to 8°C under sterile conditions after reconstitution.- 3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Applications: |
Immunochromatography (capture and conjugate);
Preparing monoclonal or polyclonal antibodies for HBsAg-preS1;
ELISA. |
