| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 4.2 kDa, a single non-glycosylated polypeptide chain containing 39 amino acids. |
| AA Sequence: |
HGEGTFTSDL SKQMEEEAVR LFIEWLKNGG PSSGAPPPS |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
1. Regulates Glucose levels rapidly;
2. Reduces Insulin resistance;
3. Reduces Glucagon;
4. Reduces HbA1c;
5. Stimulates beta cell growth which stimulates insulin production. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 10 EU/mg of rExendin-4 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Exenatide |
| Background: |
Exendin-4 is a newly discovered peptide consisting of 39 amino acids, originally found in the venom of the Gila monster Heloderma suspectum. It shows a 53% similarity in sequence with GLP-17-36amide and acts on the same membrane receptor. in vivo studies demonstrate that exendin-4 boosts insulin secretion in response to glucose levels, reduces excessive glucagon secretion, and decelerates gastric emptying. Moreover, it encourages the proliferation and formation of new ß-cells in laboratory settings and animal models. Recombinant exendin-4 is produced in Escherichia coli through the expression of a synthetic DNA sequence encoding its 39 amino acids. |
