| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
| AA Sequence: |
AAMHSVNCRL RDIYHKSLVL SGACELQAVH LNGENTNQQV VFCMSFVQGE EETDKIPVAL GLKEKNLYLS CGMKDGKPTL QLETVDPNTY PKRKMEKRFV FNKMEIKGNV EFESAMYPNW YISTSQAEKS PVFLGNTRGG RDITDFIMEI TSA |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 20 pg/mL, corresponding to a specific activity of > 5.0 × 107 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH7.4, 0.1% Tween-80. |
| Endotoxin: |
Less than 1 EU/μg of rEqIL-1β as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
IL-1 beta |
| Background: |
Interleukin-1 beta (IL-1β) is a proinflammatory cytokine that is typically produced by activated macrophages but can also come from neutrophils, epithelial cells, and endothelial cells. While it does not secrete, it does carry out metabolic and physiological functions and participates in haematopoiesis. More importantly, it plays a key role in immune response regulation. Both IL-1α and IL-1β have similar biological actions as they bind to the same receptor. The interleukin-1 beta that is recombinant in horses is a 17 kDa protein that contains 153 amino acids. It has a 63% to 70% amino acid sequence identity with IL1β in dogs, cotton rats, pigs, cats, humans, mice, rats, and rhesus monkeys. |
