| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 26.9 kDa, a single non-glycosylated polypeptide chain containing 239 amino acids. |
| AA Sequence: |
MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of reGFP as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
eGFP |
| Background: |
The green fluorescent protein (GFP), initially isolated from the jellyfish Aequorea victoria, is a 26.9 kDa protein consisting of 238 amino acid residues. It emits green fluorescence when exposed to short-wave light (from blue to ultraviolet). Beyond its wild-type form, GFP has been extensively modified through mutation for broader research applications. Enhanced GFP (eGFP) incorporates S65T and F64L mutations, which respectively enhance its fluorescence and improve its folding efficiency at 37°C. This adaptation enables GFP to be utilized effectively in mammalian cells. In A. Victoria, GFP serves as an acceptor for energy transfer. It has long been employed in cellular and molecular biology as a genetic expression reporter. Moreover, GFP functions as a molecular thermometer for precise temperature measurement in fluids. |
