| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 14.2 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. |
| AA Sequence: |
APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN INFKSFKENL KDFLFNIPFD CWKPVKK |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/mL, corresponding to a specific activity of > 2.0 × 105 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rCaGM-CSF as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α |
| Background: |
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is released by various cells in response to immune stimuli and initially identified as a factor supporting granulocyte-macrophage colony formation. It stimulates the growth and differentiation of hematopoietic precursors and has roles in non-hematopoietic cells, including inducing migration and proliferation of endothelial cells. GM-CSF also promotes the growth of certain tumor cell lines and is reported to lack cross-species biological effects. |
