| Synonym(s): |
Antigen LB39 AA antibody; Antigen LB39-AA antibody; Antigen SK29 AA antibody; Antigen SK29-AA antibody; MAR1_HUMAN antibody; MART 1 antibody; MART-1 antibody; MART1 antibody; Melan A antibody; Melan A protein antibody; Melanoma antigen recognized by T cells 1 antibody; Melanoma antigen recognized by T-cells 1 antibody; MLAN A antibody; MLANA antibody; OTTHUMP00000021036 antibody; OTTHUMP00000021037 antibody; OTTHUMP00000021038 antibody; Protein Melan-A antibody |
| Description: |
Rabbit monoclonal [EP1422Y] to MelanA |
| Application: |
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF |
| Purity: |
Protein A purified |
| Shipping: |
Shipped at 4°C. |
| Storage: |
Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer: |
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA |
| Species Reactivity: |
Reacts with: human |
| Tissue Specificity: |
Expression is restricted to melanoma and melanocyte cell lines and retina. |
| Clonality: |
Monoclonal |
| Clone Number: |
EP1422Y |
| Host: |
Rabbit |
| Isotype: |
IgG |
| Formulation: |
Liquid |
| Positive Control: |
WB: Human melanoma lysates. IHC-P: Human melanoma tissue. Flow Cyt (intra): A375 and Malme-3M cells. ICC/IF: MeWo cells. |
| Cellular Localization: |
Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation. |
| Dissociation Constant (KD): |
KD = 1.27 × 10-9 M |
| Post-translational Modifications: |
Acylated |
| Function: |
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. |
