| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Biological Activity: |
Active |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 80-235 |
| Origin: |
Rat |
| Source: |
Yeast |
| Characteristics: |
ED50 < 50 pg/mL, measured in a cytotoxicity assay using mouse L-929 cells in the presence of actinomycin D, corresponding to a specific activity of > 2.0x 107 units/mg. AA 80-235, expressed with an N-terminal Met. |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Endotoxin Level: |
< 0.2 EU/μg, determined by LAL method. |
| Format: |
Lyophilized |
| Reconstitution: |
Reconstituted in ddH2O at 100 μg/mL. |
| Buffer: |
Lyophilized after extensive dialysis against PBS. |
| Storage: |
- 80°C |
| Storage Comment: |
Lyophilized recombinant rat tumor necrosis factor-alpha (rrTNF-alpha) remains stable up to 6 months at - 80°C from date of receipt. Upon reconstitution, rrTNF-alpha should be stable up to 2 weeks at 4°C or up to 3 months at - 20°C. |
| Expiry Date: |
6 months |
| Alternative Name: |
Tumor necrosis factor alpha (TNFa) |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
Tumor necrosis factor-alpha (TNF-Alpha), also referred to as Cachectin and TNFSF1A, plays a crucial role in various physiological processes including growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases. It also contributes to the body's response against viral, bacterial, fungal, and parasitic infections. TNF is implicated not only in inducing hemorrhagic necrosis of tumors but also in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune conditions such as Crohn's disease, rheumatoid arthritis, and graft-versus-host disease.
Recombinant rat Tumor Necrosis Factor-Alpha (rrTNF-Alpha), synthesized in P. pastoris, is a glycosylated polypeptide consisting of 157 amino acids. This fully biologically active molecule possesses a molecular mass of 17.4 kDa, as determined by reducing SDS-PAGE analysis. |
| Molecular Weight: |
17.4 kDa, observed by reducing SDS-PAGE. |
