Cat. No.: | SPODRP00912 |
Size: | |
Quantity: |
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Pricey: | Inquiry |
Target: | TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
Protein Type: | Recombinant |
Protein Characteristics: | AA 80-235 |
Origin: | Rat |
Source: | E. coli |
Application: | ELISA, mass spectrometry (MS), SDS-PAGE (SDS), western blotting (WB) |
Purpose: | Recombinant Rat TNF alpha Protein is produced by E.coli expression system and the target gene encoding Leu80-Leu235 is expressed. |
Sequence: | MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL |
Purity: | Greater than 95% as determined by reducing SDS-PAGE. |
Endotoxin Level: | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Sterility: | 0.2 μm filtered |
Format: | Lyophilized |
Reconstitution: | It is not recommended to reconstitute to a concentration less than 100 μg/mL. |
Buffer: | Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
Handling Advice: | Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Storage: | - 20°C |
Storage Comment: | Lyophilized protein should be stored at - 20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2 - 8°C for 2 - 7 days. Aliquots of reconstituted samples are stable at - 20°C for 3 months. |
Alternative Name: | TNF alpha |
Synonyms: | DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |