| Target: |
IL-12 (Interleukin 12) |
| Protein Type: |
Recombinant |
| Origin: |
Rat |
| Source: |
Human cells |
| Purification tag / Conjugate: |
This IL12 protein is labelled with His tag. |
| Purpose: |
Recombinant Rat Interleukin-12/IL-12 Protein (His Tag) |
| Sequence: |
Met23-Ser335 |
| Characteristics: |
Recombinant rat interleukin-12 is produced by our mammalian expression system and the target gene encoding Met23-Ser335(p40) & Arg23-Ser215(p35) is expressed with a 6His tag at the C-terminus. |
| Purity: |
> 95% as determined by SDS-PAGE. |
| Endotoxin Level: |
< 1.0 EU per μg as determined by LAL test. |
| Format: |
Lyophilized |
| Reconstitution: |
Please refer to the printed manual for detailed information. |
| Buffer: |
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
| Storage: |
4°C, - 20°C, - 80°C |
| Storage Comment: |
Generally, lyophilized proteins are stable for up to 12 months when stored at - 20 to - 80°C. Reconstituted protein solution can be stored at 4 - 8°C for 2 - 7 days. Aliquots of reconstituted samples are stable at < - 20°C for 3 months. |
| Alternative Name: |
Interleukin-12/IL-12 |
| Synonyms: |
CLMF, CLMF2, IL-12B, NKSF, NKSF2, p40, Il-12b, Il12p40, Il-12p40, Il12, IL-12p40, IL-12, LOC100217488, IL-12p35, Il-12a, Ll12a, p35, IL12p40, IL12B, IL-12 p35, interleukin 12B, interleukin 12A, interleukin 12b, interleukin 12a, Interleukin 12 level, IL12B, IL12A, Il12b, Il12a, IL12, il12a. |
| Background: |
Interleukin 12 (IL-12) stands as the foundational member within the IL-12 family of heterodimeric cytokines, bearing significant immunological roles. It comprises two subunits linked by disulfide bonds, with weights of 35 kDa and 40 kDa, respectively. The 35 kDa subunit (p35) manifests as an α-helical protein, akin to IL-6 and GCSF. Conversely, the 40 kDa subunit (p40) boasts a fibronectin type III domain and an Ig C2-like domain, exhibiting a structural resemblance to type I cytokine receptors. While the p35 subunit is exclusive to IL-12, the p40 subunit is also found in IL-23. The mature rat p35 spans 194 amino acids (aa) and is secreted as a heterodimer fused with p40. It contains three potential N-linked glycosylation sites and shares 86% and 58% aa sequence identity with mouse and human p35, respectively. Mature rat p40, comprising 313 aa, can assume various forms including monomers, homodimers, heterodimers with p19 (resulting in IL-23), and heterodimers with p35 (forming IL-12). IL-12 plays pivotal roles in fostering hematopoietic stem cell proliferation, stimulating NK cell proliferation, and amplifying the expansion and late activation of Th1 CD4+ T cells. |
| Molecular Weight: |
36.7 & 22.6 kDa |
