| Target: |
IL-12 (Interleukin 12) |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 23-215, AA 23-335 |
| Origin: |
Rat |
| Source: |
Human cells |
| Purification tag / Conjugate: |
This IL12 protein is labelled with His tag. |
| Application: |
ELISA, mass spectrometry (MS), SDS-PAGE (SDS), western blotting (WB) |
| Purpose: |
Recombinant Rat IL-12 Protein is produced by mammalian expression system and the target gene encoding Met23-Ser335 (p40) , Arg23-Ser215 (p35) is expressed with a 6His tag at the C-terminus. |
| Sequence: |
MWELEKDVYV VEVDWRPDAP GETVTLTCDS PEEDDITWTS DQRRGVIGSG KTLTITVREF LDAGQYTCHR GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VHRNTDLKFN IKSSSSSPES RAVTCGRASL SAEKVTLNQR DYEKYSVACQ EDVTCPTAEE TLPIELVVEA QQQNKYENYS TSFFIRDIIK PDPPKNLQVK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKTK ETEEECNQKG AFLVEKTSAE VQCKGANICV QAQDRYYNSS CSKWTCVPCR GRSHHHHHH& RVIPVSGPAK CLNQSQNLLK TTDDMVRTAR EKLKHYSCTA GDIDHEDITR DKTSTLEACL PLELHKNESC LATKETSSII RGSCLPPQKT SLMMTLCLGS IYEDLKMYQS EFQAINAALQ SHNHQQITLD RNMLMAIDEL MRSLNHSGET LHQKAPMGEA DPYRVKMKLC ILLHAFSTRV MTINRVMNYL SSSHHHHHH |
| Purity: |
Greater than 95% as determined by reducing SDS-PAGE. |
| Sterility: |
0.2 μm filtered |
| Format: |
Lyophilized |
| Buffer: |
Lyophilized from a 0.2 μM filtered solution of PBS, pH7.4. |
| Handling Advice: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage: |
- 20°C |
| Storage Comment: |
Lyophilized protein should be stored at - 20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2 - 8°C for 2 - 7 days. Aliquots of reconstituted samples are stable at - 20°C for 3 months. |
| Alternative Name: |
IL12A, IL12B |
| Synonyms: |
CLMF, CLMF2, IL-12B, NKSF, NKSF2, p40, Il-12b, Il12p40, Il-12p40, Il12, IL-12p40, IL-12, LOC100217488, IL-12p35, Il-12a, Ll12a, p35, IL12p40, IL12B, IL-12 p35, interleukin 12B, interleukin 12A, interleukin 12b, interleukin 12a, Interleukin 12 level, IL12B, IL12A, Il12b, Il12a, IL12, il12a. |
