| Target: |
IL-12 (Interleukin 12) |
| Biological Activity: |
Active |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 23-215, AA 23-335 |
| Origin: |
Rat |
| Source: |
CHO cells |
| Purification tag / Conjugate: |
This IL12 protein is labelled with His tag. |
| Characteristics: |
ED50 < 0.1 ng/mL, measured in a cell proliferation assay using 2D6 cells. AA 23-215 (p35) and AA 23-335 (p40), expressed with C-terminal 6 × His. |
| Purity: |
> 95% as analyzed by SDS-PAGE. |
| Endotoxin Level: |
< 0.2 EU/μg, determined by LAL method. |
| Format: |
Lyophilized |
| Reconstitution: |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
| Buffer: |
Lyophilized after extensive dialysis against PBS. |
| Storage: |
- 80°C |
| Storage Comment: |
Lyophilized recombinant rat interleukin-12 (IL-12), His remains stable up to 6 months at - 80°C from date of receipt. Upon reconstitution, rat interleukin-12 (IL-12), His should be stable up to 1 week at 4°C or up to 2 months at - 20°C. |
| Expiry Date: |
6 months |
| Alternative Name: |
Interleukin-12 (IL-12) |
| Synonyms: |
CLMF, CLMF2, IL-12B, NKSF, NKSF2, p40, Il-12b, Il12p40, Il-12p40, Il12, IL-12p40, IL-12, LOC100217488, IL-12p35, Il-12a, Ll12a, p35, IL12p40, IL12B, IL-12 p35, interleukin 12B, interleukin 12A, interleukin 12b, interleukin 12a, Interleukin 12 level, IL12B, IL12A, Il12b, Il12a, IL12, il12a. |
| Background: |
Interleukin-12 (IL-12), alternatively known as NKSF, TCMF, CLMF, and TSF, is a heterodimeric cytokine consisting of p35 and p40 subunits. It is secreted by various immune cells including monocytes, macrophages, B cells, and dendritic cells in response to stimuli such as bacterial lipopolysaccharides and intracellular pathogens. IL-12 exerts its effects by binding to the IL-12 receptor complex, comprising IL-12 Rβ1 and IL-12 Rβ2. The actions of IL-12 include promoting the proliferation and activation of hematopoietic stem cells, natural killer cells, and T-cells. Crucially, it plays a vital role in the differentiation of Th1 cells, driving the secretion of IFN-gamma and IL-2. |
| Molecular Weight: |
26-28 kDa (p35) and 42-45 kDa (p40), observed by reducing SDS-PAGE. |
