| Application: |
Western blotting 1:2000
Simple western 1:10 - 1:50
Immunoprecipitation 1:50
Immunohistochemistry (paraffin) 1:200 - 1:800
Immunofluorescence (immunocytochemistry) 1:200 - 1:400
Flow cytometry (fixed/permeabilized) 1:800 - 1:1600 |
| Storage: |
Supplied in 10 mM sodium HEPES (pH7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Molecular Weight: |
44, 42 kDa |
| Antibody Source: |
Rabbit |
| Specificity: |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP rabbit mAb detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases. |
| Isotype: |
IgG |
| Reactivity: |
H M R Hm Mk Mi Dm Z B Dg Pg Sc |
| Sensitivity: |
Endogenous |
