| Description: |
Standard serums undergo lipid extraction to enhance clarity, followed by dialysis against phosphate buffered saline (PBS) containing sodium azide, and are subsequently freeze-dried. Diluting normal serum to 5% (v/v) in PBS is highly recommended as a blocking agent to minimize background interference caused by non-specific binding to conserved sequences and/or Fc receptors. Optimal outcomes are achieved when using diluted normal serum sourced from the same host species as the labeled antibody, applied in a separate incubation step before introducing the primary antibody. |
| Type: |
Normal Serums and Gamma Globulins |
| Formulation: |
Freeze-dried solid |
| Storage: |
Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. |
| Host: |
Goat |
| Conjugate: |
Unconjugated |
| Preservative: |
0.05% Sodium azide |
| RRID: |
AB_2336990 |
| Shelf Life: |
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use. |
| Suggested Working Concentration or Dilution Range: |
For blocking purposes, use 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instruction yields 100% serum. |
