| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 89-235 |
| Origin: |
Mouse |
| Source: |
E. coli |
| Purpose: |
Recombinant Mouse Tumor Necrosis Factor/TNFα |
| Sequence: |
MDKPVAHVVA NHQVEEQLEW LSQRANALLA NGMDLKDNQL VVPADGLYLV YSQVLFKGQG CPDYVLLTHT VSRFAISYQE KVNLLSAVKS PCPKDTPEGA ELKPWYEPIY LGGVFQLEKG DQLSAEVNLP KYLDFAESGQ VYFGVIAL |
| Characteristics: |
Recombinant mouse tumor necrosis factor/TNFα. The ED50 for this effect is typically 6.8 pg/mL. Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. |
| Purity: |
> 95% as determined by reducing SDS-PAGE. |
| Endotoxin Level: |
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test |
| Sterility: |
0.2 μm filtered |
| Format: |
Lyophilized |
| Reconstitution: |
It is not recommended to reconstitute to a concentration less than 100 μg/mL. |
| Buffer: |
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4. |
| Handling Advice: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. |
| Storage: |
4°C, - 20°C, - 80°C |
| Storage Comment: |
Lyophilized protein should be stored at < - 20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4 - 7°C for 2 - 7 days. Aliquots of reconstituted samples are stable at < - 20°C for 3 months. |
| Alternative Name: |
Tumor necrosis factor alpha (TNFa) |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
We utilize our E. coli expression system to produce Recombinant Mouse Tumor Necrosis Factor/Tnf. The target protein encompasses the sequence from Asp89 to Leu235 of Mouse Tnf. Tumor Necrosis Factor (TNF) belongs to the Tumor Necrosis Factor family and typically forms a homotrimer, interacting with SPPL2B. Predominantly secreted by macrophages, TNF can trigger cell death in specific tumor cell lines and is a pivotal cytokine implicated in the pathogenesis of various inflammatory disorders. Its influence extends to the development of type 2 diabetes, affecting insulin resistance and fatty acid metabolism. |
| Molecular Weight: |
16.4 kDa |
