| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 80-235 |
| Origin: |
Mouse |
| Source: |
Insect cells |
| Purification tag / Conjugate: |
This TNF alpha protein is labelled with rho-1D4 tag. |
| Application: |
Crystallization (Crys), ELISA, SDS-PAGE (SDS), western blotting (WB) |
| Sequence: |
LRSSSQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIALSequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. |
| Characteristics: |
From design to production - by highly experienced protein experts.Mouse Tnf protein (raised in insect cells) purified by multi-step, protein-specific process to ensure crystallization grade. State-of-the-art algorithm used for plasmid design (gene synthesis).This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein. The concentration of our recombinant proteins is measured using the absorbance at 280 nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. |
| Purification: |
Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells: Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot. |
| Purity: |
> 95% as determined by SDS PAGE, size exclusion chromatography and western blot. |
| Endotoxin Level: |
Protein is endotoxin-free. |
| Sterility: |
0.22 μm filtered |
| Grade: |
Crystallography grade |
| Format: |
Liquid |
| Buffer: |
100 mM NaCl, 20 mM hepes, 10% glycerol. pH value is at the discretion of the manufacturer. |
| Handling Advice: |
Avoid repeated freeze-thaw cycles. |
| Storage: |
- 80°C |
| Storage Comment: |
Store at - 80°C. |
| Expiry Date: |
Unlimited (if stored properly) |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
This cytokine binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. Predominantly secreted by macrophages, it can induce cell death in certain tumor cell lines. As a potent pyrogen, it causes fever directly or by stimulating interleukin-1 secretion, and it is implicated in the induction of cachexia. Under specific conditions, it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form also induces IL-12 production in dendritic cells. |
| Molecular Weight: |
18.4 kDa including tag. |
