| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Biological Activity: |
Active |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 80-235 |
| Origin: |
Mouse |
| Source: |
Baculovirus infected Insect cells |
| Purification tag / Conjugate: |
This TNF alpha protein is labelled with His tag. |
| Application: |
SDS-PAGE (SDS) |
| Sequence: |
LRSSSQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIALHHHH HH |
| Purity: |
> 90% by SDS - PAGE |
| Endotoxin Level: |
< 1.0 EU per 1ug of protein (determined by LAL method) |
| Format: |
Liquid |
| Concentration: |
1 mg/mL |
| Buffer: |
Liquid in. Phosphate buffered saline (pH7.4). |
| Handling Advice: |
Avoid repeated freezing and thawing cycles. |
| Storage: |
4°C, - 20°C, - 80°C |
| Storage Comment: |
Can be stored at + 4°C short term (1-2 weeks). For long term storage, aliquot and store at - 20°C or - 70°C. |
| Alternative Name: |
Tumor necrosis factor alpha (TNFa) |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
Tumor necrosis factor (TNF), also known as tumor necrosis factor isoform 1, is the prototypical ligand of the TNF superfamily. This pleiotropic molecule is crucial in inflammation, apoptosis, and immune system development. It is produced by various immune and epithelial cells and is assembled intracellularly into a noncovalently linked homotrimer that is expressed on the cell surface. Surface-bound TNF can induce the lysis of neighboring tumor and virus-infected cells and can trigger its own downstream signaling upon binding with soluble TNFR I. Additionally, TNF promotes inflammatory responses by activating vascular endothelial cells and macrophages. Recombinant Mouse TNF, fused to a His-tag at the C-terminus, was expressed in insect cells and purified using conventional chromatography techniques. |
| Molecular Weight: |
18.0 kDa (162aa) 18 - 28 kDa (SDS-PAGE under reducing conditions) |
