| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Protein Type: |
Recombinant |
| Protein Characteristics: |
AA 57-235 |
| Origin: |
Mouse |
| Source: |
E. coli |
| Purification tag / Conjugate: |
This TNF alpha protein is labelled with His tag. |
| Application: |
Crystallization (Crys), ELISA, SDS-PAGE (SDS), western blotting (WB) |
| Sequence: |
GPQRDEKFPN GLPLISSMAQ TLTLRSSSQN SSDKPVAHVV ANHQVEEQLE WLSQRANALL ANGMDLKDNQ LVVPADGLYL VYSQVLFKGQ GCPDYVLLTH TVSRFAISYQ EKVNLLSAVK SPCPKDTPEG AELKPWYEPI YLGGVFQLEK GDQLSAEVNL PKYLDFAESG QVYFGVIALSequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. |
| Characteristics: |
From design to production - by highly experienced protein experts.Mouse Tnf Protein (raised in E. coli) purified by multi-step, protein-specific process to ensure crystallization grade. State-of-the-art algorithm used for plasmid design (gene synthesis).This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein. The concentration of our recombinant proteins is measured using the absorbance at 280 nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. |
| Purification: |
Two step purification of proteins expressed in bacterial culture:In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot. |
| Purity: |
> 95% as determined by SDS PAGE, size exclusion chromatography and western blot. |
| Endotoxin Level: |
Endotoxin has not been removed. |
| Sterility: |
0.22 μm filtered |
| Grade: |
Crystallography grade |
| Format: |
Liquid |
| Buffer: |
100 mM NaCl, 20 mM hepes, 10% glycerol. pH value is at the discretion of the manufacturer. |
| Handling Advice: |
Avoid repeated freeze-thaw cycles. |
| Storage: |
- 80°C |
| Storage Comment: |
Store at - 80°C. |
| Expiry Date: |
Unlimited (if stored properly) |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
This cytokine binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. Predominantly secreted by macrophages, it can induce cell death in certain tumor cell lines. As a potent pyrogen, it causes fever directly or by stimulating interleukin-1 secretion, and it is implicated in the induction of cachexia. Under specific conditions, it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form also induces IL-12 production in dendritic cells. |
| Molecular Weight: |
20.7 kDa including tag. |
