| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Reactivity: |
Mouse |
| Detection Method: |
Colorimetric |
| Method Type: |
Sandwich ELISA |
| Detection Range: |
15.6 pg/mL - 1000 pg/mL |
| Minimum Detection Limit: |
15.6 pg/mL |
| Sample Type: |
Cell culture supernatant, plasma, serum |
| Analytical Method: |
Quantitative |
| Sensitivity: |
15.6 pg/mL |
| Application: |
ELISA |
| Sample Volume: |
100 μL |
| Plate: |
Pre-coated |
| Purpose: |
Sandwich ELISA for Quantitative Detection of Antigen |
| Characteristics: |
Synonyms: APC1 protein, Cachectin, DIF, soluble form, Tnf, TNF a, TNF alpha, TNF macrophage derived, TNF monocyte derived, TNF superfamily member 2, TNF-a, TNF-alpha, TNFA_HUMAN, TNFSF2, Tumor necrosis factor, Tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2Background: Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6.Gene Name: TNFProduction: Natural and recombinant Mouse TNF alpha. There is no detectable cross-reactivity with other relevant proteins.Standard: Expression system for standard: CHO, Immunogen sequence: L80-L235 |
| Handling Advice: |
Avoid cycles of freezing and thawing. |
| Storage: |
RT, 4°C, - 20°C |
| Storage Comment: |
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at - 20°C or below for 12 months. |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
