| Target: |
IL-6 (Interleukin 6) |
| Reactivity: |
Mouse |
| Detection Method: |
Colorimetric |
| Method Type: |
Sandwich ELISA |
| Detection Range: |
6.25 pg/mL - 400 pg/mL |
| Minimum Detection Limit: |
6.25 pg/mL |
| Sample Type: |
Serum, plasma, tissue homogenate |
| Analytical Method: |
Quantitative |
| Sensitivity: |
3.75 pg/mL |
| Components: |
Pre-coated, ready to use 96-well strip plate
Plate sealer for 96 wells
Standard
Sample/standard dilution buffer
Assay diluent
Biotin-labeled antibody (concentrated)
HRP-streptavidin (HRP-SA)
Biotin system (BS)
BS dilution buffer
TMB substrate
Stop solution
Wash buffer (25 x concentrate)
Instruction manual |
| Application: |
ELISA |
| Sample Volume: |
50 μL |
| Plate: |
Pre-coated |
| Purpose: |
For quantitative detection of IL-6 in serum, plasma, tissue homogenates and other biological fluids. |
| Grade: |
High sensitivity |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
For unopened kit: All reagents should be stored according to the labels on the vials. The standard and 96-well strip plate should be stored at - 20°C upon receipt, while the other reagents should be stored at 4°C.
For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper. |
| Expiry Date: |
6 months |
| Alternative Name: |
Interleukin 6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
Interleukin-6 (IL-6) is a multifaceted cytokine, characterized by its pleiotropic nature, comprising a helical structure weighing between 22 to 28 kDa. It undergoes phosphorylation and variable glycosylation. IL-6 plays a pivotal role in orchestrating various physiological processes, including the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and the progression of cancer. Together with TNF-α and IL-1, IL-6 propels the initiation of the acute inflammatory response. Particularly noteworthy is its predominant role in inducing fever and orchestrating the acute phase response primarily in the liver. Furthermore, IL-6 holds significance in the transition from acute inflammation to the development of acquired immunity or chronic inflammatory diseases. |
