| Target: |
IL-6 (Interleukin 6) |
| Reactivity: |
Mouse |
| Detection Method: |
Colorimetric |
| Method Type: |
Sandwich ELISA |
| Sample Type: |
Cell lysate, tissue lysate |
| Analytical Method: |
Quantitative |
| Sensitivity: |
2 pg/mL |
| Components: |
Pre-coated 96-well strip microplate
Wash buffer
Stop solution
Assay diluent(s)
Lyophilized standard
Biotinylated detection antibody
Streptavidin-conjugated HRP
TMB one-step substrate |
| Application: |
ELISA |
| Sample Volume: |
100 μL |
| Plate: |
Pre-coated |
| Specificity: |
The antibody pair provided in this kit recognizes mouse IL-6. |
| Purpose: |
Mouse IL-6 ELISA Kit for cell and tissue lysate samples. |
| Characteristics: |
Strip plates and additional reagents allow for use in multiple experiments.
Quantitative protein detection.
Establishes normal range.
The best products for confirmation of antibody array data. |
| Handling Advice: |
Avoid repeated freeze-thaw cycles. |
| Storage: |
- 20°C |
| Storage Comment: |
The entire kit may be stored at - 20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at - 80°C. |
| Expiry Date: |
6 months |
| Alternative Name: |
IL-6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
The mouse IL-6 ELISA kit is designed for precise quantification of mouse IL-6 levels in cell lysate and tissue lysate samples through an enzyme-linked immunosorbent assay (ELISA) conducted in vitro. This assay employs a 96-well plate coated with an antibody specifically targeting mouse IL-6. Standards and samples are dispensed into the wells, allowing IL-6 present in the sample to bind to the immobilized antibody. Following a wash step to remove unbound components, a biotinylated anti-mouse IL-6 antibody is introduced. After another wash to eliminate excess biotinylated antibody, an HRP-conjugated streptavidin solution is added to the wells. Subsequently, a TMB substrate solution is applied, leading to color development in proportion to the bound IL-6 amount. The reaction is halted with a Stop Solution, resulting in a color change from blue to yellow, and the intensity of the color is measured at 450 nm to determine IL-6 concentration.
Reproducibility:
Intra-Assay: CV < 10%.
Inter-Assay: CV < 12%. |
