| Target: |
IL-6 (Interleukin 6) |
| Protein Type: |
Recombinant |
| Origin: |
Mouse |
| Source: |
E. coli |
| Purpose: |
Recombinant Mouse Interleukin-6/IL-6 |
| Sequence: |
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT |
| Characteristics: |
Recombinant mouse interleukin-6/Il6 is produced with our E. coli expression system. The target protein is expressed with sequence (Phe25Thr211) of mouse Il6. |
| Purification: |
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test |
| Purity: |
> 95% as determined by reducing SDS-PAGE. |
| Sterility: |
0.2 μm filtered |
| Format: |
Lyophilized |
| Reconstitution: |
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Buffer: |
Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH7.4. |
| Handling Advice: |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. |
| Storage: |
4°C, - 20°C, - 80°C |
| Storage Comment: |
Lyophilized protein should be stored at < - 20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4 - 7°C for 2 - 7 days. Aliquots of reconstituted samples are stable at < - 20°C for 3 months. |
| Expiry Date: |
3 months |
| Alternative Name: |
Il6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
Interleukin-6 (IL-6) is a cytokine known for its dual role as both a pro-inflammatory agent and a crucial component of the immune system. In mice, IL-6 is directly implicated in the body's responses to infection and injury, potentially rivaling the significance of IL-1 in orchestrating the acute phase response. Various cell types, including fibroblasts, activated T cells, monocytes/macrophages, and endothelial cells, are reported to produce IL-6 in mice. Its influence extends to diverse cell types such as fibroblasts, myeloid progenitor cells, T cells, B cells, and hepatocytes. The biological functions of IL-6 are broad, such as driving the final differentiation of B-cells into Ig-secreting cells, promoting the growth of myeloma and plasmacytoma, fostering nerve cell differentiation in hepatocytes, and inducing acute phase reactants. |
| Molecular Weight: |
21.8 kDa |
