| Target: |
IL-6 (Interleukin 6) |
| Reactivity: |
Mouse |
| Detection Method: |
Colorimetric |
| Method Type: |
Competition ELISA |
| Detection Range: |
50 - 1000 pg/mL |
| Minimum Detection Limit: |
50 pg/mL |
| Sample Type: |
Serum, plasma, cell culture supernatant, tissue homogenate |
| Analytical Method: |
Quantitative |
| Sensitivity: |
1.0 pg/mL |
| Components: |
Microtiter plate (96 wells stripwell) - 1
Enzyme conjugate - 1 vial
Standard A - 1 vial
Standard B - 1 vial
Standard C - 1 vial
Standard D - 1 vial
Standard E - 1 vial
Standard F - 1 vial
Substrate A - 1 vial
Substrate B - 1 vial
Stop solution - 1 vial
Wash solution - 1 vial
Balance solution - 1 vial
Instruction manual - 1 |
| Application: |
ELISA |
| Sample Volume: |
100 μL |
| Assay Time: |
1.5 h |
| Plate: |
Pre-coated |
| Purpose: |
For the quantitative determination of mouse IL-6 concentrations in serum, plasma,cell culture supernates and tissue homogenate. |
| Characteristics: |
This ELISA kit is a solid phase ELISA designed for quantitative determination of interleukin 6. |
| Precaution of Use: |
This kit contains a small amount of 3, 3’, 5, 5’-tetramethylbenzidine (TMB) in substrate B. TMB is non-carcinogenic but it is hazardous in case of skin contact, eye contact, ingestion and inhalation. In case of contact, rinse affected area with plenty of water. The stop solution provided with this kit is an acid solution. Wear protective gloves, clothing, and face protection. Care should be taken when handling the standard because of the known and unknown effects of it. Care should also be taken to avoid contact of skin or eyes with other kit reagents or specimens. In the case of contact, wash immediately with water. Do not pipette by mouth. Avoid generation of aerosols. Waste must be disposed of in accordance with federal, state and local environmental control regulations. All blood components and biological materials should be handled as potentially hazardous. Decontaminate and dispose specimens and all potentially contaminated materials as they could contain infectious agents. The preferred method of decontamination is autoclaving for a minimum of 1 hour a 121.5°C. |
| Storage: |
4°C |
| Expiry Date: |
6 months |
| Alternative Name: |
Interleukin 6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
