| Target: |
IFNA (Interferon alpha) |
| Protein Type: |
Recombinant |
| Origin: |
Mouse |
| Source: |
HEK-293 cells |
| Sequence: |
Glu27-Thr429 |
| Purity: |
> 95% as determined by Tris-Bis PAGE, > 95% as determined by HPLC |
| Endotoxin Level: |
Less than 1EU per μg by the LAL method. |
| Sterility: |
0.22 μm filtered |
| Format: |
Lyophilized |
| Buffer: |
Lyophilized from 0.22 μm filtered solution in PBS (pH7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Handling Advice: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
| Storage: |
4°C, - 80°C |
| Storage Comment: |
Reconstituted protein stable at - 80°C for 12 months, 4°C for 1 week. |
| Expiry Date: |
12 months |
| Alternative Name: |
IFN alpha |
| Background: |
IFN-alpha/beta R1, alternatively known as IFNAR1, is a member of the class II cytokine receptor family. These receptors form heterodimeric complexes responsible for mediating signals from class II cytokines. The subunits within these receptor complexes are shared, serving various functions. Typically, IFNAR1 acts as a heterodimer alongside IFNAR2. Binding of type I interferons activates the JAK-STAT signaling pathway, leading to tyrosine phosphorylation of several proteins, including JAKs, TYK2, STAT proteins, and the alpha- and beta-subunits of the IFNR itself. |
| Molecular Weight: |
72.5 kDa. Due to glycosylation, the protein migrates to 83 - 110 kDa based on Tris-Bis PAGE result. |
