| Minimum Essential Medium Eagle (MEM), developed by Harry Eagle, is a synthetic cell culture medium tailored to approximate the protein composition of mammalian cells more closely. It features higher concentrations of amino acids compared to other media. Optional supplementation with non-essential amino acids in formulations using Hanks' or Earle's salts has expanded its versatility.
The α modification of MEM, also known as αMEM, incorporates Earle's balanced salts along with non-essential amino acids, sodium pyruvate, and additional vitamins. This enriched variation represents the most comprehensive formulation of MEM available. It includes all 21 standard amino acids, some at elevated levels, and incorporates five additional vitamins to support various cellular functions in culture. |
