| Target: |
IL-6 (Interleukin 6) |
| Reactivity: |
Human |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Conjugate: |
This IL-6 antibody is un-conjugated |
| Application: |
Immunohistochemistry (IHC), neutralization (Neut), radioimmunoassay (RIA), ELISA, western blotting (WB) |
| Specificity: |
This purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). |
| Characteristics: |
IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. |
| Immunogen: |
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with mature length recombinant human IL-6 produced in E. coli. Immunogen type: Recombinant protein. |
| Isotype: |
IgG |
| Format: |
Lyophilized |
| Concentration: |
1.0 mg/mL |
| Reconstitution: |
Reconstitution buffer: restore with deionized water (or equivalent), Reconstitution volume: 100 µL |
| Buffer: |
0.02 M potassium phosphate, 0.15 M sodium chloride, pH7.2. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at - 20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one year from date of opening. |
| Expiry Date: |
12 months |
| Alternative Name: |
IL-6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
The anti-IL-6 antibody has been confirmed through ELISA, immunohistochemistry, and western blot assays. IL-6, a secreted cytokine, exhibits diverse biological functions. It serves as a potent inducer of the acute phase response, crucial in the differentiation of B-cells into Ig-secreting cells, and plays roles in lymphocyte and monocyte differentiation. IL-6 is implicated in promoting myeloma and plasmacytoma growth, driving nerve cell differentiation, and impacting various cell types such as B-cells, T-cells, hepatocytes, hematopoietic progenitor cells, and CNS cells. Additionally, IL-6 functions as a myokine, released into the bloodstream post-muscle contraction to enhance fat breakdown and improve insulin resistance. |
