| Target: |
IL-6 (Interleukin 6) |
| Reactivity: |
Human |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Conjugate: |
This IL-6 antibody is conjugated to HRP |
| Application: |
Immunohistochemistry (IHC), neutralization (Neut), radioimmunoassay (RIA), ELISA, western blotting (WB) |
| Specificity: |
This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6. |
| Characteristics: |
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µL by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. |
| Purification: |
purified |
| Immunogen: |
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E. coli. Immunogen type: Recombinant protein. |
| Isotype: |
IgG |
| Format: |
Lyophilized |
| Concentration: |
1.1 mg/ml |
| Reconstitution: |
Reconstitution buffer: restore with deionized water (or equivalent), Reconstitution volume: 100 µL |
| Buffer: |
0.02 M potassium phosphate, 0.15 M sodium chloride, pH7.2, 10 mg/mL bovine serum albumin (BSA) - immunoglobulin and protease free. |
| Handling Advice: |
Do not add sodium azide! |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at - 20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one year from date of opening. |
| Expiry Date: |
12 months |
| Alternative Name: |
IL-6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
IL-6, a secreted cytokine, exerts diverse biological effects. It triggers the acute phase response and facilitates the maturation of B-cells into Ig-secreting cells, impacting lymphocyte and monocyte development. Additionally, IL-6 stimulates the growth of myeloma and plasmacytoma cells, fosters nerve cell differentiation, and influences various cell types including B-cells, T-cells, hepatocytes, hematopoietic progenitors, and CNS cells. Furthermore, IL-6 functions as a myokine, being released into the bloodstream post-muscle contraction. It promotes lipid breakdown and enhances insulin sensitivity. |
