| Target: |
IL-6 (Interleukin 6) |
| Reactivity: |
Human |
| Host: |
Mouse |
| Clonality: |
Monoclonal |
| Conjugate: |
This IL-6 antibody is un-conjugated |
| Application: |
Flow cytometry (FACS), immunohistochemistry (IHC), neutralization (Neut), radioimmunoassay (RIA), ELISA, western blotting (WB) |
| Specificity: |
This purified antibody detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. |
| Characteristics: |
IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Anti-IL-6 antibody antibody is ideal for investigators involved in cancer, neuroscience and immunology research. |
| Purification: |
purified |
| Immunogen: |
This protein A purified monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-6 produced in E. coli followed by hybridoma development. Immunogen type: Recombinant protein. |
| Clone: |
33A12-G9 |
| Isotype: |
IgG1 kappa |
| Format: |
Liquid |
| Concentration: |
2.19 mg/mL |
| Buffer: |
0.02 M potassium phosphate, 0.15 M sodium chloride, pH7.2. |
| Precaution of Use: |
This product contains sodium azide: a poisonous and hazardous substance which should be handled by trained staff only. |
| Storage: |
4°C |
| Storage Comment: |
Store vial at 4°C before opening. Do not freeze! This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one year from date of opening. |
| Expiry Date: |
12 months |
| Alternative Name: |
IL-6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
IL-6, a secreted cytokine, possesses a broad spectrum of biological functions. It serves as a potent stimulator of the acute phase response and plays a pivotal role in guiding the final differentiation of B-cells into Ig-secreting cells, thereby influencing lymphocyte and monocyte differentiation. Furthermore, IL-6 contributes to the growth of myeloma and plasmacytoma, as well as the differentiation of nerve cells. Its effects extend to various cell types including B-cells, T-cells, hepatocytes, hematopoietic progenitor cells, and cells of the central nervous system.
Moreover, IL-6 functions as a myokine, being released into the bloodstream post-muscle contraction. It facilitates the breakdown of fats and enhances insulin sensitivity. Researchers engaged in cancer, neuroscience, and immunology studies can benefit from utilizing anti-IL-6 antibodies in their investigations. |
