| Target: |
IL-6 (Interleukin 6) |
| Reactivity: |
Human |
| Host: |
Mouse |
| Clonality: |
Monoclonal |
| Conjugate: |
This IL-6 antibody is un-conjugated |
| Application: |
ELISA |
| Specificity: |
Cross-reactivity with IL-1alpha, IL-1beta, IL-4, IL-8, IL-18. |
| Purification: |
Protein A affinity column |
| Immunogen: |
Human IL-6 recombinant protein |
| Clone: |
2F6H6 |
| Isotype: |
IgG2b kappa |
| Format: |
Lyophilized |
| Concentration: |
0.5 mg/mL |
| Reconstitution: |
Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL. |
| Buffer: |
Lyophilized with PBS, pH7.4, containing 0.02% sodium azide. |
| Precaution of Use: |
Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Handling Advice: |
Avoid repeated freezing and thawing. |
| Storage: |
- 20°C |
| Storage Comment: |
The antibody is stable in lyophilized form if stored at - 20°C or below. The reconstituted antibody can be stored for 2 - 3 weeks at 2 - 8°C. For long term storage, aliquot and store at - 20°C or below. |
| Alternative Name: |
IL-6 |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
| Background: |
Interleukin 6 (IL6) is a versatile 24 kDa protein initially identified in the culture medium of RNA-stimulated fibroblastoid cells. Its expression is boosted by various factors like IL1, TNF, PDGF, IFN beta, TNF alpha, NGF, and IL17, while it is suppressed by glucocorticoids, IL4, and TGF beta. IL6 plays a direct role in orchestrating responses following infections and cellular damage, potentially rivaling the significance of IL1 and TNF alpha in regulating the acute phase response. Moreover, IL6 is implicated in modulating adipose tissue mass. It is generated by fibroblasts, activated T cells, monocytes or macrophages, and endothelial cells. Acting on multiple cell types such as fibroblasts, myeloid progenitors, T cells, B cells, and hepatocytes, IL6 is pivotal in numerous physiological processes. Additionally, it collaborates with IL2 in promoting T lymphocyte proliferation and enhances the proliferative effects of IL3 on multipotential hematopoietic progenitors. |
