| Target: |
IL-18 (Interleukin 18) |
| Binding Specificity: |
AA 175-193, C-term |
| Reactivity: |
Human |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Conjugate: |
This IL-18 antibody is un-conjugated |
| Application: |
ELISA, western blotting (WB) |
| Purpose: |
Rabbit IgG polyclonal antibody for Interleukin-18(IL18) detection. Tested with WB in Human. |
| Sequence: |
KKEDELGDRS IMFTVQNED |
| Characteristics: |
Rabbit IgG polyclonal antibody for interleukin-18(IL18) detection. Tested with WB, ELISA in human.
Gene name: interleukin 18(interferon-gamma-inducing factor).
Protein name: interleukin-18. |
| Purification: |
Immunogen affinity purified. |
| Immunogen: |
A synthetic peptide corresponding to a sequence at the C-terminus of human IL18 (175-193aa KKEDELGDRSIMFTVQNED). |
| Isotype: |
IgG |
| Format: |
Lyophilized |
| Concentration: |
500 μg/mL |
| Reconstitution: |
Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL. |
| Buffer: |
Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg thimerosal, 0.05 mg sodium azide. |
| Precaution of Use: |
This product contains sodium azide and thimerosal (merthiolate): poisonous and hazardous substances which should be handled by trained staff only. |
| Handling Advice: |
Avoid repeated freezing and thawing. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
At - 20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at - 20°C for a longer time. |
| Expiry Date: |
12 months |
| Alternative Name: |
IL18 |
| Synonyms: |
Igif, Il-18, IL-18, IGIF, ChIL-18, IL-1g, IL1F4, IL18, interleukin 18, Il18, IL18. |
| Background: |
Interleukin-18 (IL-18), also referred to as IL18, is a proinflammatory cytokine encoded by the IL18 gene in humans. It belongs to the IL-1 superfamily and is chiefly produced by macrophages and various other cells. IL-18 functions by binding to the interleukin-18 receptor, cooperating with IL-12 to trigger cell-mediated immunity upon microbial product infection like lipopolysaccharide (LPS). Upon IL-18 stimulation, natural killer (NK) cells and certain T cells release interferon-gamma (IFN-gamma), also known as type II interferon, crucial for activating macrophages and other cells. This cytokine, when combined with IL-12, has been observed to suppress IL4-dependent IgE and IgG1 production while enhancing IgG2a production in B cells. Additionally, IL-18 binding protein (IL18BP) can interact specifically with IL-18, thus dampening its biological activity. The human IL18 gene maps to 11q22.2-q22.3, closely linked to the DRD2 gene locus and distinct from mapped IDDM loci. |
